Electron Microscopy (EM) Facility

The Electron Microscopy (EM) Facility at the CDR, headed by Dr. Chris Goulbourne is a core facility that provides EM service to investigators. The facility houses a ThermoFisher Talos L120C TEM with 2D imaging capability, dual axis electron tomography for 3D ultrastructural evaluation and cryo EM. Services offered include ultrastructural evaluation in 2D and 3D, protein localization using immunogold techniques and cryogenic evaluation of proteins, viruses, and cellular architecture. Additionally, the facility offers guidance and sample preparation for employing correlative light and electron microcopy (CLEM) and nano secondary ion mass spectrometry (NanoSIMS) for tracking stable isotope tagged molecules. Sample preparation instrumentation includes a Leica UCT ultramicrotome and ThermoFisher Mark IV Vitrobot, as well as a dedicated high-performance workstation for processing 3D data sets using Amira and Imod software. The services of the facility are currently available to external academic institutes and industrial facilities on a fee-for-use-basis. Additional information can be obtained by contacting Dr. Chris Goulbourne at Chris.Goulbourne@NKI.rfmh.org.